Diagnostic Tests with Flow Cytometry

Qualitative and Quantitative Analyses (Tests) and applications by flow cytometry techniques for many Medical specialties and basic sciences

Tests

(LOW COST ESTIMATION DEPENDING ON AMOUNT OF SAMPLES for international purposes)

1- Complete Immunophenotype

Quantitative detection of 6 subpopulations of lymphocytes, which includes numbers of cells and percentages: B, T, NK, NKT, CD4, CD8

The sample is collected in EDTA tube (3ml) and sent by Courier to the lab within 2 days.

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Application: For general investigation of the immune system from physicians, clinical immunologists, gynecologists (infertility and RSAs), pediatricians, pneumonologists, hematologists, neurologists, rheumatologists etc.

2- Detection of levels of Τ, ΝΚ, ΝΚΤ cells

The sample is collected in EDTA tube (3ml) and sent by Courier to the lab within 2 days.

3- Detection of sperm DNA FRAGMENTATION(DFI)

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4-Count of absolute number of white blood cells and number of spermatozoa

5- Estimation of sperm viability

6- Estimation of acrosomal protein in the surface and inside the sperm

For tests 3) to 6) sperm is required in cryovials and to be shipped in dry ice. These specific tests are performed on males who appear fragmented DNA (3), and this factor is responsible for women’s infertility and RSAs

In test (6) the sperm capability of penetration through zona pellucida is detected by flow cytometry.

Application: For general investigation of sperm from gynecologists, anthologists, IVF centers etc.

7- Quantitative detection of platelet functionality

This detection includes estimation per cell of the following gycoproteins PAC-1, CD41, CD42a, CD42b, CD61, CD62P, GPIIb-IIIa

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Application: For the investigation of people who have a tendency for thrombosis or general in thromboembolic diseases from cardiologists, physicians, haematologists etc.

8- Detection of allergic factors by the basophil degranulation test

The sample is collected in heparin Na tube 3 ml and shipped by Courier to the lab within 2 days.

Application: For the investigation of people who have a tendency in allergic reactions from pediatricians, immunologists allergologists etc.

9- Functional insufficiency of phagocytosis

The sample is collected in heparin Na tube 3 ml and shipped by Courier to the lab within 2 days.

Application: For the investigation of people who have a tendency in decrease immune response from pediatricians, immunologists allergiologists etc.

10- Chronic lymphoproliferative disorders – Lymphomas

The sample is collected in EDTA 3 ml and shipped directly by Courier to the lab within 2 days..

Application: For the investigation of people who are suspicious for LYMPHOPROLIFERATIVE syndromes from haematologists.

11- Paroxysmal nocturnal hemoglobinuria (PNH) 

The sample is collected in EDTA 3 ml and shipped directly by Courier to the lab within 2 days.

Application: For the investigation of people who are suspicious for myelodysplastic (MDS) syndromes from haematologists.

12- Immunophenotype for diagnosis in immunodeficiencies) SCID 

The sample is collected in EDTA 3 ml and shipped directly by Courier to the lab within 2 days.

Application: For the investigation of people who are suspicious for immunodeficiencies (SCID from immunologists, pediatricians, hematologists, neurologists, rheumatologists.

Detection of the following (SURFACE ANTIBODIES): CD3, CD4, CD8, CD16, CD56, CD19, CD20, CD40L, HLA-DR, TCRαβ, TCRγδ, CD45RO, CD45RA, CD62L, CD25, CD69, CD27, CD28, CD80, CD86.

13- Estimation of soluable molecules (proteins, cytokines) in serum of plasma with the use of beads

(very important protocol for infamation and chronic diseases and for detoxification of the matrix in regulating physiology) 

TH1/TH2/ΤΗ17/ΤΗ22 (IL-1b, IL-2, IL-4, IL-5, IL-6, IL-9,IL-10, IL-12p70,  IL-13, IL-17, IL-22, TNF-a, IFN-γ)

The above cytokines in serum or cellular levels execute the most important properties of the human physiology

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14- Specific research protocols

(CYTOPLASMIC CYTOKINES):

IL-2, IL-4, IL-5, IL-6, IL-10, INF-γ, TNFa.

QUANTITATION OF SURFACE RECEPTORS:

CD38, CD20

DNA ANALYSIS 
CELL DETECTION ACCORDING TO PHASE

1. RELAXATION (G0/G1)
2. SYNTHESIS DNA (S)
3. MITOSIS (G2/M)

DNA INDEX Τιμή 150

DETECTION OF: DNA INDEX

DETECTION OF APOPTOSIS

DETECTION OF CHANGES IN CELLULAR MEMBRANE

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ΑΝΝΕΧΙΝ V, PROPIDIUM IODIDE, APO-1, CD95.
ANALYSIS OF ENDOCELLULAR ORGANELLES br> BCL-2

Investigation of Caspases

ESTIMATION OF ABSOLUTE NUMBER OF CD34+ (STEM CELLS)
CD45/CD34 WITH PROCOUNT KIT